Impact of Magnesium and Vitamin D Supplementation on Serum Antimullerian Hormone and Follicle Stimulating Hormone in Women with Infertility
DOI:
https://doi.org/10.65761/pjcr.2026.336Keywords:
Infertility, Anti-Müllerian Hormone, Follicle-Stimulating Hormone, Magnesium, Vitamin D SupplementsAbstract
Background: Infertility is influenced by multiple reproductive, endocrine, metabolic, and nutritional factors. Anti-Müllerian hormone (AMH) and follicle-stimulating hormone (FSH) are established biochemical markers used in the assessment of ovarian function. Evidence regarding the effects of combined magnesium and vitamin D supplementation on these markers in women with infertility remains limited.
Objective: To evaluate the association of 60 days of combined magnesium and vitamin D supplementation with changes in serum AMH and FSH concentrations among infertile women aged 25–35 years.
Methods: This single-arm, quasi-experimental pre–post intervention study included 40 infertile women aged 25–35 years. Participants received magnesium 150 mg/day and cholecalciferol 2000 IU/day for 60 consecutive days. Serum AMH and FSH were measured before supplementation and after completion of the intervention during the early follicular phase. Paired-samples t-tests were used to compare baseline and post-intervention concentrations.
Results: Mean serum AMH increased from 0.898 ± 0.226 to 1.137 ± 0.235 ng/mL, representing a mean increase of 0.239 ng/mL (95% CI: 0.164–0.315; t(39) = −6.392; p < 0.001). Mean FSH decreased from 11.925 ± 0.987 to 11.720 ± 1.000 mIU/mL, with a mean reduction of 0.205 mIU/mL (95% CI: 0.174–0.236; t(39) = 13.514; p < 0.001). Significant AMH increases and FSH reductions were observed in both age groups.
Conclusion: Sixty days of combined magnesium and vitamin D supplementation was associated with increased AMH and modestly reduced FSH concentrations in infertile women aged 25–35 years. Controlled randomized trials are required to determine causality and clinical fertility relevance.
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